Síndrome metabólica ou diabetes acentua os sintomas semelhantes ao transtorno do estresse pós-traumatico e a ativação glial

Abstract

The psychiatric alteration triggered after a traumatic experience or event is characterized as Post-traumatic Stress Disorder (PTSD). Studies have shown that PTSD can modify the metabolism, but if the altered metabolism can modify its development is still not clear. Thus, the present study aimed to investigate the influences of the development of PTSD on metabolic alterations, such as in the metabolic syndrome (MS) and diabetes. Experiment 1: the characterization of MS was investigated in animals that ingested a 10% fructose solution or diabetes in animals that received a single dose of alloxan (150 mg / kg) intraperitoneally. Experiment 2: after characterization (MS or diabetes) the groups of animals were exposed or not to inescapable shock and social isolation (14 days) and were evaluated in the following behavioral tests: open field, social interaction test, forced swimming, elevated plus maze and object recognition test and in the verification of protein quantification (Western Blotting technique). In addition, the verification of protein quantification (Western Blotting technique) to evaluate the expression of glial acid fibrillar protein (GFAP) and ionized calcium binding adapter molecule 1 (Iba-1) in the hippocampus and pre-frontal cortex. The results demonstrate that PTSD MS and PTSD Control animals group an increase in freezing time, increase immobility in the forced swimming test (suggesting a depressive behavior), increase time in closed arms and reduced time in open arms (suggesting behavior anxious type), reduced in the social interaction and memory deficit when compared to their respective controls (unexposed control and unexposed MS). The PTSD Diabetes and PTSD Control animals group showed similar results to those found in animals with MS when compared to their respective controls (Non-PTSD Control and Non-PTSD Diabetes). In addition, it is noteworthy that these changes were more pronounced in PTSD MS and PTSD Diabetes animals compared to their respective PTSD Controls. Regarding glial activation, was observed an increased expression of GFAP and Iba-1 in MS PTSD and increased expression of GFAP in Diabetes PTSD animals group. Therefore, it is possible to conclude that MS or diabetes pre-existing accentuates symptoms related to PTSD. And that in animals with PTSD MS the changes observed may be related to glial activation.