Estudo fitoquímico, avaliação da atividade analgésica e antiinflamatória de extratos e substâncias isoladas de Arrabidaea brachypoda (DC.) Bureau (Bignoniaceae)

Abstract

In Brazil, several species of Bignoniacea have medicinal and some biological activities have been proven experimentally, such as anti-tumor activity, anti-inflammatory and antimicrobial. Naphthoquinones, lignans, triterpenes and flavonoids were previously isolated from species of this family. However, the chemical composition and biological activities of most of them remain unknown. This paper aims to study the chemical and evaluation of analgesic and anti-inflammatory of roots, stems, leaves and isolated compounds from Arrabidaea brachypoda. For this study were obtained the hexanic, chloroformic, ethylic acetate and ethanolic extracts from different parts of the plant for phytochemical studies, in addition to ethanolic extracts for pharmacological evaluations. The compounds were identified by comparison with authentic standards, melting temperature and the usual spectroscopic techniques. The anti-inflammatory activity was evaluated in the model of paw edema, peritonitis and LPS-induced granuloma by comparing the results with the control groups. For tests of analgesia were used the following animal models: abdominal writhing, formalin, tail-flick "and" hot plate ". We compare the results with the patterns: positive (morphine) and negative (CMC). The results presented in the test paw edema for the extracts and the substance RABEC-1 showed a reduction in paw edema of mice in treated groups compared to control groups. In the granuloma test, there was a reduction in the formation of granulomatous tissue in animals treated with ethanol extract of the root and also a reduction in the number of leukocytes in the trial of peritonitis induced by LPS. In the model of analgesia was observed that extracts of root and leaf showed peripheral analgesic activity while the compound RABEC-1, showed activity central and peripheral. results indicate that extracts of root and leaf and the substance isolated from A. brachypoda tested, may be a potential target for use in therapy of pain and inflammation. This work thus contributes to the knowledge of chemistry and pharmacological activity of A. brachypoda.